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不同发酵阶段的灵芝菌丝三萜类成分与抗肿瘤作

关键字: 不合发酵阶段的灵芝菌丝三萜类因素与抗肿瘤感化的相关性钻研 文章择要: 不合发酵阶段的灵芝菌丝三萜类因素与抗肿瘤感化的相关性钻研CORRELATIONBETWEENINTRACELLULARTRITERPENESFROMMYCELIAOFGANODERMALUCIDUMINDIFFERENTGROWTHSTAGEANDINHIBITIONEFFECTONTUMORCELLS余素萍 张劲松 唐庆九 史先敏 刘艳芳 杨焱 潘�...

不合发酵阶段的灵芝菌丝三萜类因素与抗肿瘤感化的相关性钻研

CORRELATION BETWEEN INTRACELLULAR TRITERPENES FROM MYCELIA OF GANODERMA LUCIDUM IN DIFFERENT GROWTH STAGE AND INHIBITION EFFECT ON TUMOR CELLS

余素萍 张劲松 唐庆九 史先敏 刘艳芳 杨焱 潘迎捷 

摘 要:本文钻研了不合培养要领、不合发酵阶段的菌丝三萜各组分的变更与抗肿瘤感化的关系.结果注解灵芝三萜在菌丝发酵后期才大年夜量孕育发生,菌丝中三萜的种类与子实体比拟相对较少,不合发展阶段菌丝中的三萜在组分、相对含量及各组分间的比例都有所变更,此中两个组分峰的增高与其对肿瘤细胞的抑制感化反相关,另有几个组分峰的增高与对肿瘤细胞的抑制感化正相关.本钻研确定了临盆有效抑制肿瘤细胞发展的三萜组分最佳培养要领和最佳培养光阴.

关键词:灵芝,胞内三萜,培养要领,HPLC,抗肿瘤感化

分类号:Q939.97  文献标识码:A

文章编号:1672-6472(2004)04-0548-0554

作者简介:张劲松,通讯作者

作者单位:余素萍(南京农业大年夜学微生物学系,南京,210095;上海农科院食用菌钻研所;上海市农科院天然药用资本钻研与开拓中间;上海市农业遗传育种重点开放实验室农业部食用菌遗传育种重点开放实验室,上海,201106) 

张劲松(上海农科院食用菌钻研所;上海市农科院天然药用资本钻研与开拓中间;上海市农业遗传育种重点开放实验室农业部食用菌遗传育种重点开放实验室,上海,201106) 

唐庆九(上海农科院食用菌钻研所;上海市农科院天然药用资本钻研与开拓中间;上海市农业遗传育种重点开放实验室农业部食用菌遗传育种重点开放实验室,上海,201106) 

史先敏(南京农业大年夜学微生物学系,南京,210095;上海农科院食用菌钻研所;上海市农科院天然药用资本钻研与开拓中间;上海市农业遗传育种重点开放实验室农业部食用菌遗传育种重点开放实验室,上海,201106) 

刘艳芳(上海农科院食用菌钻研所;上海市农科院天然药用资本钻研与开拓中间;上海市农业遗传育种重点开放实验室农业部食用菌遗传育种重点开放实验室,上海,201106) 

杨焱(上海农科院食用菌钻研所;上海市农科院天然药用资本钻研与开拓中间;上海市农业遗传育种重点开放实验室农业部食用菌遗传育种重点开放实验室,上海,201106) 

潘迎捷(南京农业大年夜学微生物学系,南京,210095;上海农科院食用菌钻研所;上海市农科院天然药用资本钻研与开拓中间;上海市农业遗传育种重点开放实验室农业部食用菌遗传育种重点开放实验室,上海,201106) 

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收稿日期:2004年5月13日

修稿日期:2004年7月10日

出版日期:2004年11月22日

菌物学报

MYCOSYSTEMA

2004 Vol.23 No.4 P.548-554

    

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